Poly(A)Purist MAG Kit from Thermo Fisher Scientific

Product Specs
ItemPoly(A)Purist MAG Kit
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberAM1922
FormatKit
TargetmRNA
Brand/TrademarkInvitrogen™
TypeMAG Kit
ApplicationsMicroarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Sample TypeRNA (Total)
Time45 min

Add to Compare List

Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Cell autonomous role of leucine-rich repeat kinase in protection of dopaminergic neuron survivalbioRxivDecember 5, 2023Jongkyun Kang, Guodong Huang, Long Ma, Youren Tong, Anu Shahapal, Phoenix Chen, Jie ShenNorthern analysis, polyA+ RNA was enriched from ~500 μg total RNA using the Poly(A)Purist™ MAG Kit (AM1922, ThermoFisher) according to the manufacturer’s instruction. For the LRRK2, ~10 μg of total RNA was used

(2) Hidden evolutionary constraints dictate the retention of coronavirus accessory genesbioRxivOctober 20, 2023Stephen A. Goldstein, Teagan M. Feeley, Kristina M. Babler, Zoë A. Hilbert, Diane M. Downhour, Niema Moshiri, Nels C. Eldemaximize yield. Polyadenylated RNA was extracted using the Invitrogen Poly(A)Purist MAG Kit (Cat # AM1922) following the manufacturer protocol. 100 ng PolyA+ RNA was prepared for sequencing using the Direct

(3) Swi/Snf Chromatin Remodeling Regulates Transcriptional Interference and Gene RepressionbioRxivApril 30, 2023Kaitlin Morse, Sarah Swerdlow, Elçin Ünaldirect mRNA-seq Poly(A) selection was performed on 600 μg of RNA using the Poly(A)Purist™ MAG kit (AM1922, Ambion) following manufacturer’s instructions. Poly(A) RNA was precipitated at −20°C for 18 hours in

(4) Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeuticsNature CommunicationsDecember 1, 2022Kathrin Leppek et al.µL of 500 mM of Na-EDTA. Quenched samples were purified with 2 µL of Oligo dT bead (Thermo Fisher, AM1922) and 0.8 µL of 10 µM FAM-A20-Tail2. RNA, bead, and primer were incubated at room temperature for 15

(5) Enhanced transcriptome-wide RNA G-quadruplex sequencing for low RNA input samples with rG4-seq 2.0BMC BiolNovember 13, 2022Jieyu Zhao, Eugene Yui-Ching Chow, Pui Yan Yeung, Qiangfeng Cliff Zhang, Ting-Fung Chan, Chun Kit Kwok(Qiagen, 74136). PolyA RNAs were purified using the Poly(A) PuristMAG Kit (Thermo Fisher Scientific, AM1922). After 2 rounds of selection, the corresponding amount of polyA RNA was incubated with 1× fragmentation

(6) Platr4 is an early embryonic lncRNA that exerts its function downstream on cardiogenic mesodermal lineage commitmentDevelopmental cellNovember 7, 2022Hazra R, Brine L, Garcia L, Benz B, Chirathivat N, Shen MM, Wilkinson JE, Lyons SK, Spector DL.P3 Strain CF-1, Irradiated",Millipore,Cat # R-4169444.2 Poly(A)Purist™ MAG Kit,Thermo Fisher,Cat # AM1922 Proteinase K Solution,Viagen Biotech,Cat # 501-PK Iscove’s Modification of DMEM,Corning,Cat # 15-016-CV Ham’s

(7) SIRPα maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosisEBioMedicineNovember 1, 2022Wang D, Lin Y, Xu F, Zhang H, Zhu X, Liu Z, Hu Y, Dong G, Sun B, Yu Y, et al.RNA was purified using the Poly(A) Purist kit (Ambion, Thermo Fisher Scientific, Waltham, MA, USA, ##AM1922) according to the manufacturer's instructions. A total of 5 μg of poly(A) RNA was recovered, and a cDNA

(8) Development of a ribosome profiling protocol to study translation in Kluyveromyces marxianusFEMS Yeast ResearchSeptember 12, 2022Darren A Fenton, Stephen J Kiniry, Martina M Yordanova, Pavel V Baranov, John P Morrisseychemistry. Alternatively, RNA-Seq was carried out using polyA selection using Poly(A)Purist Mag Kit (#AM1922, Ambion) as per the manufacturer's instructions. PolyA selected cDNA library was generated and sequenced

(9) Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeuticsbioRxivSeptember 5, 2022Kathrin Leppek et al.μL of 500 mM of Na-EDTA. Quenched samples were purified with 2 μL of Oligo dT bead (Thermo Fisher, AM1922) and 0.8 μL of 10 μM FAM-A20-Tail2. RNA, bead, and primer were incubated at room temperature for 15

(10) Compatibility rules of human enhancer and promoter sequencesNatureJuly 7, 2022Drew T. Bergman, Thouis R. Jones, Vincent Liu, Judhajeet Ray, Evelyn Jagoda, Layla Siraj, Helen Y. Kang, Joseph Nasser, Michael Kane, Antonio Rios, et al.this kit has been discontinued, we now use the Poly(A)Purist MAG kit from Thermo Fisher Scientific, AM1922). Following mRNA elution, we treated with TURBO DNase (Thermo Fisher Scientific, AM2238) in 100uL reactions

(11) Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencingSTAR ProtocolsMarch 18, 2022Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünalcommercial assays,Critical commercial assays,Critical commercial assays ,, Poly(A)Purist MAG kit,Ambion,AM1922 KAPA HyperPrep Kit,Roche,KK8504 Direct RNA Sequencing Kit,Oxford Nanopore Technologies,SQK-RNA002 ,, Deposited

(12) High-resolution analysis of cell-state transitions in yeast suggests widespread transcriptional tuning by alternative startsGenome BiologyDecember 1, 2021Minghao Chia, Cai Li, Sueli Marques, Vicente Pelechano, Nicholas M. Luscombe, Folkert J. van Wervenfrom each sample. At least 5 μg of mRNAs was purified from total RNA using the Poly(A)Purist MAG kit (AM1922, Ambion). mRNAs were fragmented, and fragments were dephosphorylated and then subjected to a decapping

(13) Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based techniqueRNA (New York, N.Y.)November 1, 2021Martinez Campos C, Tsai K, Courtney DG, Bogerd HP, Holley CL, Cullen BR.further 24 h. Total poly(A) RNA was purified using magnetic beads [Poly(A)Purist MAG Kit, Invitrogen #AM1922] following the manufacturer's instructions. The immunoprecipitation (IP) was performed using an antipseudouridine

(14) Core promoter activity contributes to chromatin-based regulation of internal cryptic promotersNucleic Acids ResearchAugust 20, 2021Bo Bae Lee, Hyeonju Woo, Min Kyung Lee, SeoJung Youn, Sumin Lee, Jae-Seok Roe, Soo Young Lee, TaeSoo Kimphenol/chloroform (1:1) (Sigma). 10 μg of total RNA or 1 μg of mRNA purified with Poly(A)Purist™ MAG Kit (Thermo, AM1922) was separated on agarose gel. The RNA was then transferred onto membranes (Bio-Rad, 1620159) and hybridized

(15) Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification SequencingMethods in Molecular BiologyJune 26, 2021Bryan R. Cullen, Kevin Tsai70% Ethanol GlycoBlue Coprecipitant (Invitrogen # AM9515) Poly(A)Purist MAG Kit (Invitrogen #AM1922) 2.2 Immunoprecipitation & Crosslinking IPP buffer: 10mM Tris-HCl pH7.4, 150mM NaCl, 0.1% NP-40, mix

(16) Analysis of RNA conformation in endogenously assembled RNPs by icSHAPESTAR ProtocolsJune 18, 2021Lu Chen, Howard Y. Chang, Steven E. ArtandiKit,NEB,T2040L Zero Blunt™ TOPO™ PCR Cloning Kit,Thermo/Invitrogen,450245 Poly(A)Purist™ MAG Kit,Thermo/Invitrogen,AM1922 ,, Deposited data,Deposited data,Deposited data ,, icSHAPE data,This study,GSE97486 ,, Experimental models

(17) Small RNAs are modified with N-glycans and displayed on the surface of living cellsCellJune 10, 2021Ryan A. Flynn et al.Clean and Concentrator 5,Zymo Research,Cat# R1013 Poly(A)Purist MAG Kit,Thermo Fisher Scientific,Cat# AM1922 ,, Deposited data,Deposited data,Deposited data ,, Input- and ManNAz-seq from HeLa cells,This Study,Gene Expression

(18) Integrated genomic analysis reveals key features of long undecoded transcript isoform-based gene repressionMolecular cellMay 20, 2021Tresenrider A, Morse K, Jorgensen V, Chia M, Liao H, van Werven FJ, Ünal E.(Wu et al., 2018). At least 5 μg of mRNA was purified from total RNA using the Poly(A)Purist MAG kit (AM1922, Ambion). mRNAs were fragmented for 3 minutes at 70°C using a Zinc-based alkaline fragmentation reagent

(19) Atypical E2Fs either Counteract or Cooperate with RB during Tumorigenesis Depending on Tissue ContextCancers (Basel)April 23, 2021Eva Moreno, Shusil K. Pandit, Mathilda J. M. Toussaint, Laura Bongiovanni, Liesbeth Harkema, Saskia C. van Essen, Elsbeth A. van Liere, Bart Westendorp, Alain de BruinCA, per condition. To deplete for ribosomal RNA, 5ug of total RNA was used to isolate Poly(A) USA, AM1922) followed by purification using mRNA only eukaryotic mRNA isolation RNA using (A)purist MAG kit (Life Technologies, AM1922) purification

(20) Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseasesNature communicationsApril 20, 2021Zhang M, Li K, Bai J, Velema WA, Yu C, van Damme R, Lee WH, Corpuz ML, Chen JF, Lu Z.enrichment mRNA was enriched from total crosslinked RNA using Poly(A)Purist™ MAG Kit (Invitrogen, AM1922), according to the manufacturer’s instructions. Viral RNA enrichment Total RNA (200 μg) extracted

Be the First to Write a Review!

Poly(A)Purist MAG Kit from Thermo Fisher Scientific

Description

Thermo Fisher Scientific

Citations (52)

Reviews

Poly(A)Purist MAG Kit from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map